Cloning of protein A DNA to OmpA constructs

1. Digest of pKS-A plasmid, pACYC177+OmpA_omega and pACYC177+OmpA_alpha with NotI and SacI (pACYC177 were also dephosporylated with CIAP)
2. Gel electrophoresis and gel-out of proper bands (420 bp - pKS-A lane, 4050 bp - pACYC177+OmpA_omega lane and 4300 bp pACYC177+OmpA_alpha lane).
3. Ligation of A DNA fragment with both pACYC177 vectors.
4. Transformation of E. coli TOP10 strain with ligations.
5. Transformants plating on LB + kanamycin.

Paweł: pET15b vector transformed into TOP10 strain for plasmid purification