Piotr:
1. Preparation of electro- and chemocompetent bacteria E. coli TOP10.
2. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320) on LB broth with proper antibiotics.
Michał L.:
1. Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA.
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