Michał K.

Preparation of constructs with OmpA protein fusions

1. Transformation of E. coli TOP10 strain with ligation from previous day
2. Transformants plating on LB + kanamycin

Preparation of construct pKS with A protein

1. Inactivation of digestion enzymes and CIAP
2. Ligation of digested PCR product and pKS 2h at room temperature
3. Transformation of E. coli TOP10 strain with 7 µl of ligation mix
4. Transformants plating on LB + ampicillin + X-gal + IPTG