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Team:UNIPV-Pavia/Protocols/Transformation
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Transformation
(estimated time: 3 hours and 30 min + 12-14 hours overnight incubation)
Materials needed:
- LB agar plates with proper antibiotic added incubated at 37°C
- Thawed Invitrogen TOP10 cells (every tube contains approximately 60 )
- Resuspended DNA
- SOC medium
- Cut paper spots using scalpel and tweezers (or punch tool, following provided instructions); if you use scalpel and tweezers, try to cut pieces of about the same dimension of the punch tool.
- Clean scalpel and tweezers (or punch tool) with water and ethanol every time you cut a spot; be careful to dry your tools correctly, especially if you use punch tool, which needs more time to dry than scalpel/tweezers.
- Put the cut paper into the 0.5 ml tube.
- Incubate at 42°C for 20 min.
- Vortex and spin down.
