Team:Warsaw/Calendar-Main/21 July 2008

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Cloning of protein A DNA to OmpA constructs
1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used: AL+SacI and AP+NotI
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin.
3. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_alpha).
4. Control digest of isolated plasmids with BamHI and SacI .

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Ligations transformed into TOP10 and plated on LB + ampicillin


    Cloning of protein A DNA to pET15b+Omp-alfa plasmid
    Antoni

    <p>
    1. Transformation of E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with friday ligation.
    2. Transformants plating on LB + ampitilin.
    </p>


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