Team:Warsaw/Calendar-Main/29 July 2008

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1. Separete transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.
2. Inoculation of pACYC177+OmpA_alpha and pACYC177+OmpA_omega - liquid LB with kanamycin.


Oczyszczanie białek: A-alfa, Z-alfa i Z-omega

Piotr zaszczepienie A-alfa, Z-alfa i Z-omega z chodowli nocnej do świeżego LB, chodowanie do OD=0,5 indukcja rużnymi stężeniami IPG w 22 i 37C - pobranie próbek po 3h i następnego dnia rano (próbki zamrożono)


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