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Team:Warsaw/Calendar-Main/27 August 2008
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Interactive list of topics
Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni
Digest
pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha
dephosphorylation with CIAP
Gel electrophoresis of digested plasmids and
gel-out
of proper bands
Ligation
of pET15b+alfa and A
Transformation of
E. coli
TOP10
.
Transformants plating on LB + ampicillin.