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From 2008.igem.org

Attribution analyzer / Interactive list of topics   1. Isolation of plasmids from cultures inocluated on previous day. 2. Control digest of isolated plasmids (pACYC177+omega_deltaA) with HindIII and SacI (7 from 8 colonies confirmed). Piotr Checking if OmpA_omega_A_alpha gives ampicillin resistance Inoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 into the same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100) in relation 1:50 Bacterial growth (OD) measurement in the evening: ampicillin concentration (μg/mL): IPTG concentration (mmol/mL): 0 0,1 0,25 0,5 0,75 1 25 1,054 1,154 1,051 0,99 1,096 0,896 50 0,94 0,891 1,123 0,847 0,924 0,81 75 0.5 0,63 0.743 0.782 0.631 0.64 100 0.02 0,396 0.563 0.678 0.602 0.611 Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases. (present in top10) Transformation omega_A_alpha and A_alpha into Rosetta.

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