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Team:UNIPV-Pavia/Protocols/Digestion
From 2008.igem.org
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|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
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The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- Antarctic Phosphatase
- Ligation
- PCR
BioBrick digestion with restriction enzymes
(estimated time: 2 hours and 15 min)
Materials needed:
- Roche
- Pre-warmed at 37°C bath
- Cut and gel-extracted vector
