PCRs for fusions
Michał K.

1. PCR - translation fusion: AID + T7 RNA-polymerase
   Primers:
   
   • AIDlNrH
   • T7pXbSal
   Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
   Annealing temperature : 73°C
   Elongation time: 4 minutes
   20 cycles
   
2. Gel electrophoresis and isolation of proper band (3300 bp).
3. Electrophoresis to estimate the concentration of isolated DNA.

Michał L., Ewa, Marcin:

Overnight inoculation and induction:
Top10 + 0,5 mM IPTG
pMPM-AID + Glc + 0,01% L-Ara
pTrc99a-AID + Glc + 0,5 mM IPTG