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Team:University of Lethbridge/Notebook/Project4October
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October 6, 2008
Jaden
Objective: Grow up cells containing the xylE gene from the glycerol stocks and plates (Selina had transformed with DH5a) in LB + Amp liquid media. The gene was sent to us in pVL1392 (modified pUC8 for viral expression, with Amp resistance).
Subcultured four tubes: Two from glycerol stocks and two from colonies on a plate.
October 7, 2008
Christa, Munima
Objective: Plasmid prep the xylE gene that Jaden subcultured yesterday.
Christa: Plasmid prepped one subculture tube from 'glycerol stock' and 'plate' using the Eppendorf FastPlasmid Mini Prep Kit. Stored 50 uL aliquots in the -20 C iGEM freezer box (iGEM Plasmids) labeled "xylE from glycerol stock" and "xylE from plate", and have a big blue "P" or "G" on the top, respectively.
Munima: Plasmid prepped one subculture tube from 'glycerol stock' and 'plate' using the QiaPrep Spin MiniPrep Kit. Plasmids are stored in 50 uL aliquots in the -20 C iGEM freezer in the iGEM Plasmids box. They are labeled "xylE plasmid; plate 2; Oct. 7/08" and "xylE plasmid; glycerol 2; Oct. 7/08".
