Team:Warsaw/Calendar-Main/26 June 2008
From 2008.igem.org
Michał K. Michał L., Ewa, Marcin |
Product | Templates | Primers | Product length |
---|---|---|---|
Alpha-A fusion | Alpha-linker + linker-A | AlphaL+SacI + AP+NotI | 1500 bp |
Omega-A fusion | Omega-linker + linker-A | OmegaL+SacI + AP+NotI | 1440 bp |
PCL program for fusion A with alpha and omega
Temperature | Time | |
---|---|---|
94°C | 4:00 | |
94°C | 0:30 | 28 cycles |
48°C-62°C | 0:45 | |
68°C | 2:30 | |
68°C | 10:00 | |
4°C | infinite |