Team:KULeuven/12 August 2008

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  Input Output Filter InverTimer Reset Cell Death Memory

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Lab Work

Wet Lab

• The liquid cultures of the colonies with ligation products we prepared yesterday were MiniPrepped today. After that, we did a PCR to check the ligations.
• The liquid cultures of parts F1610 and P1010 were also MiniPrepped.
• We made electrocompetent Top10 cells and tested them with PUC.
• The cells with parts C0060 + B0015, C0040 + B0015, J23100 + B0032 we electroporated yesterday gave colonies. They were streaked out and a liquid culture was made.
• The PCR of the T7 polymerase was continued.
• Some more cutting was done.

Dry Lab

Modeling

Great news today: issues from yesterday seem to be solved! Some more inaccuracies were found, but in a misterious way, they seem to have cancelled out each other in the new model. also, we included the latest version of the memory (hopefully this time the final version) in the full model without problems.

In the afternoon the good spirit was brought back to zero by the discovery of some incinsistencies with the modeling of the T7 promotor. New research had to be done and this resulted in depressing simulation results: a background noise level which completely overwhelmed our system.

A good sleep will bring more insight and better results!

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