Team:Warsaw/Calendar-Main/9 July 2008

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Preparation of constructs with OmpA protein fusions

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_omega and pACYC177+OmpA_alpha Primers used: OmpaL_N and OmpaP_link
  2. Confirmed transformant colonies inoculated to liquid LB with kanamycin

Preparation of construct pKS with A protein

  1. Colony PCR on colonies from plates with transformations pKS-A
    Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin