Team:Warsaw/Calendar-Main/22 July 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Isolation of plasmids from cultures inocluated on previous day
  2. Control digest of isolated plasmids with NdeI and NotI. Zero positive results obtained - just empty vectors
  3. Another overnight ligation of pET15b-OmpA-omega (NdeI/NotI cutted) with protein Z DNA


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