Team:Warsaw/Calendar-Main/23 August 2008

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Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni

  1. Digest pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI (Orange buffer), pET15b+OmpA_alpha dephosphorylation with CIAP
  2. Gel electrophoresis of digested plasmids and gel-out of proper bands
  3. Overnight ligation of pET15b+alfa and A



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