| Read | Date | Plasmid | Clone # | Oligo | Result | File
|
| MEA001 | 06/15/08 | pBca1256-K112200 | 1 | ca998 | Bad read | MEA001
|
| MA001 | 06/18/08 | pBca1256-K112200 | 2 | ca998 | Point mutation changing Asn to Lys | MA001
|
| MEA002 | 06/15/08 | pBca1256-K112201 | 1 | ca998 | Point mutation changing Asn to Lys | MEA002
|
| MA002 | 06/18/08 | pBca1256-K112201 | 2 | ca998 | Point mutation changing Asn to Lys | MA002
|
| MEA003 | 06/15/08 | pBca1256-K112202 | 1 | ca998 | Perfect | MEA003
|
| MEA004 | 06/15/08 | pBca1256-K112203 | 1 | ca998 | Perfect | MEA004
|
| MEA005 | 06/15/08 | pBca1256-K112204 | 1 | ca998 | Point mutation changing Asn to Lys | MEA005
|
| MA003 | 06/18/08 | pBca1256-K112204 | 2 | ca998 | Bad Read | MA003
|
| MEA006 | 06/15/08 | pBca1256-K112207 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA006
|
| MEA006 | 06/18/08 | pBca1256-K112207 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA006
|
| AL305 | 06/23/08 | pBca1256-K112207 | 1 | G00101 | Bad reverse read, 2 templates | AL305
|
| MEA007 | 06/15/08 | pBca1256-K112208 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA007
|
| MEA007 | 06/18/08 | pBca1256-K112208 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA007
|
| MA212 | 06/23/08 | pBca1256-K112208 | 1 | G00101 | Perfect read, can see silent mutation from AGG to AGA seen in forward read | MA212
|
| MEA008 | 06/15/08 | pBca1256-K112209 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA008
|
| MEA008 | 06/18/08 | pBca1256-K112209 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA008
|
| MA209 | 06/23/08 | pBca1256-K112209 | 1 | G00101 | Perfect reverse read, able to see the silent mutation from AGG to AGA seen in forward read | MA209
|
| MEA009 | 06/15/08 | pBca1256-K112210 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA009
|
| MEA009 | 06/18/08 | pBca1256-K112210 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA009
|
| MA211 | 06/23/08 | pBca1256-K112210 | 1 | G00101 | Bad reverse read, two templates present | MA211
|
| MEA010 | 06/15/08 | pBca1256-K112211 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA010
|
| MEA010 | 06/18/08 | pBca1256-K112211 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA010
|
| MA210 | 06/23/08 | pBca1256-K112211 | 1 | G00101 | Perfect reverse read, can see silent mutation from AGG to AGA seen in forward read | MA210
|
| MEA011 | 06/15/08 | pBca1256-K112212 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA011
|
| MEA011 | 06/18/08 | pBca1256-K112212 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA011
|
| MA208 | 06/23/08 | pBca1256-K112212 | 1 | G00101 | Bad reverse read, two templates present | MA208
|
| MEA012 | 06/15/08 | pBca1256-K112213 | 1 | ca998 | Perfect | MEA012
|
| MEA013 | 06/15/08 | pBca1256-K112214 | 1 | ca998 | Perfect | MEA013
|
| MEA014 | 06/15/08 | pBca1256-K112215 | 1 | ca998 | Perfect | MEA014
|
| MEA015 | 06/15/08 | pBca1256-K112216 | 1 | ca998 | Bad read in the beginning, re-sequence & pick new colony | MEA015
|
| MEA015 | 06/18/08 | pBca1256-K112216 | 1 | ca998 | This one was perfect except it shows a deletion of A in EcoRI site, but that's probably just a problem with the sequencing data | MEA015
|
| MA005 | 06/18/08 | pBca1256-K112216 | 2 | ca998 | Perfect | MA005
|
| MEA016 | 06/15/08 | pBca1256-K112217 | 1 | ca998 | Perfect match to K112218 | MEA016
|
| MEA016 | 06/18/08 | pBca1256-K112217 | 1 | ca998 | Perfect match to K112218 | MEA016
|
| MEA017 | 06/15/08 | pBca1256-K112218 | 1 | ca998 | Perfect match to K112217 | MEA017
|
| MEA017 | 06/18/08 | pBca1256-K112218 | 1 | ca998 | Perfect match to K112217 | MEA017
|
| MEA219 | 06/20/08 | pBca1256-K1122219 | 1 | ca998 | Perfect | MEA219
|
| MEA220 | 06/20/08 | pBca1256-K112220 | 1 | ca998 | Perfect, but there's an additional BamHI site inserted after the first one... | MEA220
|
| MEA018 | 06/15/08 | pBca1256-K112221 | 1 | ca998 | Perfect | MEA018
|
| MEA019 | 06/15/08 | pBca1256-K112222 | 1 | ca998 | Perfect | MEA019
|
| MEA223 | 06/20/08 | pBca1256-K112223 | 1 | ca998 | Perfect | MEA223
|
| MEA224 | 06/20/08 | pBca1256-K112224 | 1 | ca998 | Perfect | MEA224
|
| MEA020 | 06/15/08 | pBca1256-K112225 | 1 | ca998 | Perfect | MEA020
|
| MEA021 | 06/15/08 | pBca1256-K112226 | 1 | ca998 | Perfect | MEA021
|
| MEA022 | 06/15/08 | pBca1256-K112227 | 1 | ca998 | Perfect | MEA022
|
| MEA023 | 06/15/08 | pBca1256-K112228 | 1 | ca998 | Perfect match to K112229 | MEA023
|
| MEA024 | 06/15/08 | pBca1256-K112229 | 1 | ca998 | Bad read, re-sequence | MEA024
|
| MA004 | 06/18/08 | pBca1256-K112229 | 2 | ca998 | Insertion of an A... pick a new colony | MA004
|
| DV5 | 06/20/08 | pBca1256-K112229 | 3 | ca998 | Perfect match to K112230 (aside from a deletion of an A in the EcoRI site... probably just due to bad reading. Don't worry about it) | DV5
|
| MEA025 | 06/15/08 | pBca1256-K112230 | 1 | ca998 | Perfect match to K112228 | MEA025
|
| MEA026 | 06/24/08 | pBca1256-K112200 | 1 | ca998 | Perfect | MEA026
|
| MEA027 | 06/24/08 | pBca1256-K112201 | 1 | ca998 | Fatal point mutation from GCG to GTG (Ala to Val) | MEA027
|
| MEA028 | 06/24/08 | pBca1256-K112204 | 1 | ca998 | Mutation in a~ region from tgcg to tgtg, and fatal deletion of G. | MEA028
|
| MEA029 | 06/24/08 | pBca1256-K112205 | 1 | ca998 | Perfect | MEA029
|
| MEA030 | 06/24/08 | pBca1256-K112207 | 1 | g00101 | Perfect | MEA030
|
| MEA031 | 06/24/08 | pBca1256-K112210 | 1 | g00101 | Perfect | MEA031
|
| MEA032 | 06/24/08 | pBca1256-K112212 | 1 | g00101 | Perfect | MEA032
|
| MEA033 | 06/26/08 | pBca1256-K112201 | 2 | ca998 | Perfect | MEA033
|
| MEA034 | 06/26/08 | pBca1256-K112201 | 3 | ca998 | Deletion of GCGATGT | MEA034
|
| MEA035 | 06/26/08 | pBca1256-K112204 | 2 | ca998 | Perfect | MEA035
|
| MEA036 | 06/26/08 | pBca1256-K112204 | 3 | ca998 | Insertion of GCGATGT | MEA036
|
| Molly6 | 07/22/08 | pBjh1601KA-K112208 | 2 | ca998 | Perfect | Molly6_ca998
|
| Molly6 | 07/22/08 | pBjh1601KA-K112208 | 2 | G00101 | Perfect | Molly6_G00101
|
| Molly7 | 07/22/08 | pBjh1601AC-K112230 | 2 | ca998 | Sequenced perfectly for a~pelB (K112229) | Molly7
|
| Molly13-2 | 07/23/08 | pBjh1601KA-K112235 | 2 | ca998 | Perfect | Molly13-2_ca998
|
| Molly13-2 | 07/23/08 | pBjh1601KA-K112235 | 2 | G00101 | Perfect | Molly13-2_G00101
|
| Molly15-1 | 07/23/08 | pBjh1601AK-{rbs.ompT}{<int!} | 1 | ca998 | Perfect | Molly15-1
|
| Molly18-5 | 07/23/08 | pBjh1601CA-{rbs.phoApp}{<ihfB!} | 5 | ca998 | Perfect, but then we realized the wrong part was made initially. | Molly18-5
|
| Molly17-5 | 07/24/08 | pBjh1601KC-{rbs.lamB}{<ihfA!} | 5 | ca998 | Perfect, but then we realized the wrong part was made initially. | Molly17-5
|
| ig9 | 07/25/08 | pBjh1601AK-K112205 | 1 | ca998 | Perfect | ig9
|
| Molly2-2 | 07/25/08 | pBjh1601CA-K112212 | 2 | ca998 | Perfect sequence to a~int! (K112211) | Molly2-2
|
| Molly#3 | 07/26/08 | pBjh1601AK-K112236 | 3 | ca998 | Perfect, shows deletion in Kan part of vector | Molly#3
|
| 234-CK-1 | 08/05/08 | pBjh1601CK-K112234 | 1 | ca998 | Perfect | 234-CK-1_ca998
|
| 234-CK-1 | 08/05/08 | pBjh1601CK-K112234 | 1 | G00101 | G00101 site deleted in Kan part of vector, bad read | 234-CK-1_G00101
|
| 234-CK-2 | 08/05/08 | pBjh1601CK-K112234 | 2 | ca998 | Perfect | 234-CK-2_ca998
|
| 234-CK-2 | 08/05/08 | pBjh1601CK-K112234 | 2 | G00101 | G00101 site deleted in Kan part of vector, bad read | 234-CK-2_G00101
|
| 234-1256-3 | 08/08/08 | pBca1256-K112234 | 3 | ca998 | Perfect | 234-1256-3_ca998
|
| 234-1256-3 | 08/08/08 | pBca1256-K112234 | 3 | G00101 | Perfect | 234-1256-3_G00101
|
| 237-CA-1 | 08/08/08 | pBjh1601CA-K112237 | 1 | mea010 | Perfect | 237-CA-1_mea010
|
| MEA037 | 10/07/08 | pK112247 | 1 | ca1246F/R | AttL1 looks good, but attL2 looks like it might have a mutation from G->A... | MEA037
|
| MEA038 | 10/07/08 | pK112247 | 2 | ca1246F/R | Both attL1 and attL2 sites look good | MEA038
|
| MEA039 | 10/07/08 | pK112248 | 1 | ca1246F/R | Both attL1 and attL2 sites look good | MEA039
|
| MEA040 | 10/07/08 | pK112248 | 2 | ca1246F/R | Both attL1 and attL2 sites look good | MEA040
|
| MEA041 | 10/07/08 | pK112245 | 3 | JH126F | attR1 site looks good | MEA041
|
| MEA042 | 10/07/08 | pK112245 | 4 | JH126F | attR1 site looks good | MEA042
|
| MEA043 | 10/07/08 | pK112246 | 1 | JH126F | attR1 site looks good | MEA043
|
| MEA044 | 10/07/08 | pk112246 | 5 | JH126F | attR1 site looks good | MEA044
|
| MEA045 | 10/10/08 | pK112128 | 1 | JH126R | AttR2 site looks fine | MEA045
|
| MEA046 | 10/10/08 | pK112245 | 4 | JH126R | Mutation in attR2 site | MEA046
|
| MEA047 | 10/10/08 | pK112246 | 5 | JH126R | Mutation in attR2 site | MEA047
|
| MEA048 | 10/10/08 | pK112128 | 2 | JH126R | AttR2 site looks fine | MEA048
|
| MEA049 | Date | Plasmid | Clone # | Oligo | Result | MEA049
|
| MEA050 | Date | Plasmid | Clone # | Oligo | Result | MEA050
|
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