Cloning of protein A DNA to GeneArt plasmid

Antoni

1. Colony PCR on colonies from plates with transformations pGeneart+A.
   Primers used: AP+NotI and AL+SacI.
2. Gel electrophoresis.
3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.
4. Inoculation to liquid LB with ampicillin: pET15b+OmpA-alpha.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with FastBamHI and FastSacI (Fast Digest buffer).
3. Gel electrophoresis - no proper clones founded.