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Team:Warsaw/Calendar-Main/29 July 2008
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Attribution analyzer
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Interactive list of topics
Cloning of omega_A DNA fragment to
pACYC177+OmpA_alpha
Michał K.
Isolation
of
pKS+omega_A
from culture inoculated on previous day.
Digest
of
pKS+omega_A
with SacI and NotI (BamHI buffer).
Digest
(SacI and NotI) and
dephosphorylation
(CIAP) of
pACYC177+OmpA_alpha
.
Gel electrophoresis and
gel-out
of 4300 bp (
pACYC177+OmpA_alpha
) and 550 bp (omega_A) bands.
Ligation
of DNA fragments from 2. and 3. (1 hr)
Transformation
of E. coli
TOP10
strain with ligation.
Transformants plating on LB + kanamycin.
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