Team:Warsaw/Calendar-Main/2 September 2008

From 2008.igem.org

Revision as of 20:57, 19 October 2008 by PPrzanowski (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Purification of proteins: A-alpha

Piotr, Emilia

  1. pET15b+A_alpha in Rosetta strain from overnight culture inoculated in fresh LB and cultured until OD=0,5.
  2. Culture induced with different concentrations of IPTG in 22°C and 37°C.
  3. Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/2_September_2008"