Team:Warsaw/Calendar-Main/21 May 2008

From 2008.igem.org

Revision as of 11:39, 19 October 2008 by PPrzanowski (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Inoculation of separate transformant colonies for transcriptional fusion (liquid LB + tetracycline).
  2. Inoculation of separate transformant colonies for translational fusion (liquid LB + tetracycline).

Results of rifampicin test

Michał L., Ewa, Marcin

StrainInducer / repressorNumber of colonies on rifampicin
Top10none12
Top100.5 mM IPTG17
Top10 pMPM-AIDnone13
Top10 pMPM-AID0.1% Glucose18
Top10 pMPM-AID0.1% Arabinose54
Top10 pTRC-AIDnone14
Top10 pTRC-AID1% Glucose17
Top10 pTRC-AID0.5 mM IPTG53

Conclusion: AID seems to work!

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/21_May_2008"