Team:Warsaw/Calendar-Main/25 August 2008

From 2008.igem.org

Revision as of 18:23, 20 October 2008 by PPrzanowski (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Tests for ampicillin resistance given by protein added to medium

Piotr

Purification Omp_alpha of antibiotic resistance contamination

Inoculation of E. coli TOP10 careing pACTC177+OmpA_alpha.

Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Antoni

  1. Transformation of E. coli TOP10 strain with friday's ligation products.
  2. Transformants plating on LB + ampicillin.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

Eight colonies from each plate (pACYC177+OmpA_alpha_A_omega and pACYC177+OmpA_alpha_Z_omega) was inoculated to liquid LB with kanamycin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/25_August_2008"