Mutagenesis of protein A

Paweł

Treatment of mutageneses as on 23rd September.

Preparation of alpha_A construct

Antoni

1. PCR on alpha_linker and linker_A with AlphaL+SacI and AP+NotI primers and 10% DMSO (30 cycles, elongation 60 s, annealing temperature 72°C).
2. Gel electrophoresis of PCR products and gel-out of proper band (1019 bp).
3. Measurment of concentration of both isolated products.

Preparation of BioBricks

Piotr

1. E. coli TOP10 electroporation with overnight ligation of RFP(??????) and deltaA DNA fragments.
2. Plating on LB + ampicillin.