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1. Making a DNA agarose gel
From 2008.igem.org
Making the Agarose Gel (for a 1%gel 50mL volume)
1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder. 2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling). 3. Add 1.5µL of Ethimium Bromide and swirl until a fine mixture is seen. 4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends. 5. Wait for the gel to solidify over time. 6. Once hardened, remove the comb and load the gel into the electrophoresis box.
