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Team:UCSF/Willis Wong Notebook
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Willis Wong Notebook
Overall Objective:
Devise an array of inputs that cab be linked to regulation of silencing.
Milestones:
1. Define minimal fragment for light inducible dimerization.
2. Define the configuration of dimerization/regulatory domains that will yield desired function. (Silencing /
Unsilencing)
Work Done:
Week 1-2: Design AB and BD parts for combinatorial cloning (includes PhyB and Pif3 parts).
Week 3-4: Ligate AB + BD parts into 315 vectors.
Week 4-5: Subclone Pif3 constructs into 303 vectors for intregration into a yeast reporter strain [B3(H)].
Week 7-9: Transform the PhyB constructs into the yeast strains that were integrated with Pif3.
Week 10-11: In the dark, added PCB, pulsed with respective light, ran FACs.
