Team:Imperial College/Summary

The Imperial College 2008 team chose to take the engineering approach to the biofabricator project. This framework has helped us organised the project and team.

In order to achieve our specifications of design previously described, we require the following devices;

• Light sensing device - Converting a light input into a PoPS output,
• Biomaterial production device- Converting a PoPS input into an output of biomaterial production,
• Motility Control device - Converting a PoPS input into an output of motility arrest,
• Integration device - To allow integration and selection of our genetic constructs and devices into B,subtilis,

Each of these constructs makes up the final device which is shown below:

(AB is our antibiotic resistance cassette, ytvA is the gene controlling the light-sensing pathway, SB is the biomaterial, epsE the clutch and the 5' and 3' sections are integration sites. Light-inducible promoters are labelled with an 'L')

Growth Curve

Genetic Circuit

Motility Analysis

Following the design stage of our project we moved on to the implementation stage. This involved construction of a cloning strategy, construction of our biobricks and transformation and characterisation of these biobricks in B.subtilis. For more information on this aspect of the project please see the Wet Lab Hub

The wet lab team was responsible for designing the constructs and setting up the cloning strategy to get us from the starting parts to the finished system. We were also responsible for designing and BioBricking the starting parts, of course, and designing and implementing the integration brick technique. This section shows some of the major results that came from the wet lab over the summer. Motility results can go in the dry lab overview above.

Transformation

Transformation stuff goes here...

Calibration Curve

Calibration curve results go here...

Stuff

• Helped Bristol by sending them a mini-iGEM project: Chemotactic dot-to-dot with information on quorum sensing and directed movement
• Helped Bristol by sending them a part (BBa_J37015) from our 2007 stock which was an empty vector in the Registry
• Developed integration bricks, to allow devices to be constructed that can then be excised and planted into B. subtilis
• Layed the groundwork for future teams to work with B. subtilis by BioBricking promoters, RBSs, terminators and so on and characterising them
• Showed that expansion into other organisms is a definite possibility!

Of course, that's a very simplified description of our project. We expanded upon our project by looking into possible areas for real-world application; for a case-study of such an implementation check out how our project fits in with >>> Biocouture >>>