Team:Chiba/Calendar-Home/18 September 2008

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17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature
    37℃ and 30℃


Team:Output


SAP 1μl
->37℃　1hour
->30min　65℃
Zymo ->25μl
Ligation
vector:BBa_I7100
insert:BBa_S03119(ptet+LuxR),Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)

Sample No Vector&insertVector
DNA(vector) 3.53.5
DNA(insert) 7.5-
Ligase 11
Ligase Buffer 44
dH2O 44
TOTAL 2020


TRANSFORMATION
BBa_J54103(RBS+GFP)
BBa_P2006

Gel check
Ligation product(Venus YFP)
BBa_J37033
BBa_J37020
BBa_J37032

Sample No each
DNA each
Loading Dye 1
dH2O 4
TOTAL 6
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