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Team:NTU-Singapore/Notebook/29 May 2008
From 2008.igem.org
Wednesday
- ChinChong (~~)
- Requested E coli strain B2B110 with the ColE7 plasmid from Professor David Gordon (Australia)
- Requested quotation from Invitrogen on some chemicals and kits
- Discussed possible experiments design to patch up the parts of our BioBrick
- Finding parameters for the modeling from literature
- Darius
- Come up with provisional LacI experiment design
- Discussed possible experiments design to patch up the parts of our BioBrick
- Choon Kit
- Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI
- Alias with Professor Yuan Hanna (Taiwan)to get E7+Imm plasmid, expected arrival 5th Jun 08
- Alias with Professor Chris Viogt (UCSF) to get AND modular plasmids, expected arrival 5th Jun 08
- Requested full DNA sequence of ColE7 and Imm plasmid from Zhong Zhi Hao (Taiwan)
- Requested full DNA sequence of AND modular plasmids from Chris Voigt (UCSF)
- Documented laboratory work for 28 and 29 May 2008
- Hung
- Plasmid Extraction using Qiagen Miniprep. Ligation using EcoRI and PstI (basically phase II and up to step 3 of phase III of experiment 3 for iron promoter. We left the DNAs incubated overnight instead of for 2hrs due to time constraint.)
- Finding parameters for the modeling from literature
- ZhenFu
- Improve our group's model with some literature parameters
- Added equations to our model
- Puzzled over results of simulation, shall work on it further with other members of the group
