Tuesday 3 June

Chin Chong (~~)

• • Confirmed the orders for the oligo/primers designs to be purchase from 1st Base
  • List of things to purchase modified
  • Updated time-line for ordering chemicals and consumables
  • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA

ZhenFu

• • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
  • Modified model to due to more estimation of parameters

Darius

• • Printed and pasted the Grand master plan of our project on the wall
  • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA

Min Lin

• • Send out enquiries for potential sponsors
  • Waiting aproval for Proposal

Hung

• • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
  • Processing and storage of amplified DNA plasmids
  • Updated Wiki website

Choon Kit

• • Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
  • Processing and storage of amplified DNA plasmids
  • Document laboratory reports for 02 Jun 2008

Lab details

Personnel

• Hung,Choon Kit,Zhen Fu:
  • Morning:
    1. Take out from the incubator 4 cryotubes. After spinning down, the tubes were observed:
       1. 2 tubes with LBA contained no cell pellets at all as expected.
       2. 2 tubes with LB without Amp. had cell pellets at the bottom. We then poured out the liquid and the tubes at -20 oC fridge for future use.
    2. Take out from the incubator 5 agar plates. All had cell colonies growing on them except for LBA BW-W3110 plate. We stored them at 4 oC fridge.
  • Afternoon:
    1. Inoculation to obtain 4 cryotubes:
       1. 2 containing LBA with BBa_B0015 plasmid cells
       2. 2 containing LBA with LacI-GFP plasmid cells

Incubate 4 tubes overnight