Team:Chiba/Sender experiments

From 2008.igem.org

Revision as of 06:57, 25 October 2008 by Masahiro (Talk | contribs)

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Notebook

Project Design ( Sender experiments | Receiver experiments ) | Our Goal


Quorum-Sensing Cross-talk

Fig.5 Senders crosstalk
  • Senders
    • [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]

Tet-lasi chiba.gif

    • [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]

Tet-rhli chiba.gif

    • [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]

Tet-luxi chiba.gif

  • Receiver
    • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Reporter 9002 chiba.gif

Method

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 37°C or 30°C.
  5. Measured intensity of green fluorescence at regular time intervals.

Result

Home The Team The Project Parts Submitted to the Registry Notebook
Retrieved from "http://2008.igem.org/Team:Chiba/Sender_experiments"