Team:Warsaw/Calendar-Main/2 July 2008

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Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

  1. Gel electrophoresis and gel-out of proper bands
  2. Ligation of pZC320 with standard parts: BBa_E0840(Gfp genetrator) and BBa_J04450 (RFP generator)
  3. Chemotransformation of E.coli TOP10 with ligation products
  4. Plating transformants on LB+Amp30+X-gal+IPTG

    Preparation of constructs with OmpA protein fusions

    Paweł

    1. Clean-up of overnight digest.
    2. Ligation of OmpA_alfa and pET15b.
    3. Ligation of OmpA_omega and pET15b.
    4. Transformation of E. coli TOP10 strain with ligation products.
    5. Transformants plating on LB + ampicillin.