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Team:UC Berkeley/Assembly
From 2008.igem.org
Revision as of 06:27, 25 October 2008 by ChrisBrown (Talk | contribs)
Layered assembly is a straight-forward and robust method for combining two or more basic parts into a destination vector. There are three layers (Entry, Assembly and Destination).
The Entry plasmid contains the basic part, flanked by EcoRI, BglII and BamHI restriction sites and a Spectinomycin antibiotic resistance marker.
The entry plasmid is transformed into a cell that is engineered to methylate BamHI or BglII restriction sites. The part that is first
There are six plasmids in the assembly layer, each containing two antibiotic resistance markers separated by a XhoI restriction site.
