Team:Warsaw/Calendar-Main/19 June 2008

From 2008.igem.org

Revision as of 12:32, 25 October 2008 by Emilia (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Blue/white test

Piotr

  • The analysis of lacZ sequence from pZC320:
    There is no mutation in beta-galactosidase gene. Why are the colonies white?
  • Colony PCR.
    Template: DNA isolated from white colonies
    Primers: pZCseqL and pZCseqR

    Colony PCR program
    TemperatureTimeNo. of cycles
    94°C4:00
    94°C0:3028 cycles
    48°C0:45
    72°C1:30
    72°C10:00
    4°Cinfinite


    		•

    Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/19_June_2008"