User:Bipedalape/1 July 2008

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PCR: MioC (Promoter Region from JM109)

PCR settings: 95 3min {95 0.5 min 50 0.5 min 72 1 min)

(Repeated X 30)

72 10 min 4 hold

PCR Product of ~300bp (Successful) w/o DMSO


Verification of Antibiotic Plates Potency: Colonies were isolated on Chloro, Kana, Amp, and Tet. Successful