Team:Tokyo Tech/Construction

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Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry Result The Team Notebook

Construction

For confirming pressure-response ability of pressure-inducible promoter, we experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]).

fig1.Ptet-GFP
fig1.Promoter less-GFP
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