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Team:Utah State/Project
From 2008.igem.org
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Abstract
The Utah State University iGEM team project is focused on creating an efficient system for production and monitoring PHA production in microorganisms. One goal of our research is to develop and optimize a method, using fluorescent proteins, for the detection of maximum product yield of polyhydroxybutyrate (PHB, a bioplastic) in recombinant E. coli and in Cupriavidus necator. In order to develop an optimal PHB detection system, we focused on the identification of the most efficient reporter genes, and the best promoter sequences that would allow our reporter to indicate when PHB production was maximized.
Introduction
Polyhydroxybutyrate
PHB Metabolic Pathways
Problems with PHB
Green Fluorescent Protein
The Green Fluorescent Protein (GFP) used primarily used in this project was GFP E0240 plasmid from the newer iGEM paper.
