Team:Warsaw/Calendar-Main/21 October 2008

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Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 16 October) to LB with kanamycin.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day (pSB2K3 + alpha_linker under PT7 (BBa_K103019)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found Fig. 1.
Fig. 1. EcoRI/PSTI digest of pT7_alpha_link 1-3. EcoRI/PstI digests of pT7_alpha_link
3. Marker

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Inoculation of few more colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.
  2. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 +AID under pBAD/araC (BBa_K103002).
  3. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found Fig. 2.
Fig. 2. EcoRI/PstI digests of BBa_K103002
1. Marker
2-3. EcoRI/PstI digests of BBa_K103002

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K.

Inoculation of colonies from plate with ligation (pSB2K3 + OmpA_linker_alpha_linker (BBa_K103014)) to LB with kanamycin.

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