EPF-Lausanne/27 October 2008

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Cloning

  • Cloning of CD+ into AB+

First we tried to use AB+ as the insert, but then realized that AB+ and its vector both had almost equal sizes (around 2kb). It was therefore not possible to separate them using gel purification.

Fortunately, CD+ has 2.3kb, so we insert CD+ into AB+.

Again we use the modified ligation protocol:

    • Protocol:
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