Team:University of Ottawa/5 June 2008

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Contents

Today in the Lab

Dan, Matt

Digestion
  • We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
    • Gel Results
  • Gel was unsuccessful for 1A but results for other plasmids worked well.
    • Miniprep
  • We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
  • Colonies were then inoculated using LB + Amp solution and left overnight.
    • Glycerol Stock
  • Glycerol Stock was performed for plasmids 7/8 and 0.
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