The overnight PCR reaction failed.
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.
The digestion was verified on a gel and extracted using the Qiagen kit.
This time we obtained enough insert to do a ligation but not enough vector.
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C Team Fungus:
"
