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Team:University of Alberta/Plant Project
From 2008.igem.org
Contents |
Introduction
To date, synthetic biology has focused largely on single cell organisms. The goal of our project is to build BioBrick to facilitate application of synthetic biology principles to the genetic engineering of higher plants. We are developing standardized, open source binary vectors, promoters, and resistance genes for use in plants, each of which is to conform to BioBrick specifications. The application of the BioBricks in transformation of protoplasts and whole plants of tobacco and Arabidopsis, respectively, will be demonstrated, as well as a general strategy for increasing the general availability of synthetic biology tools for plant genetic engineering.
IGEM's First Binary Vector
Our Binary Vector is a modified pCambia 0380 binary vector link We have added in our own poly linker which includes the proper prefix, suffix and primers to coincide with the IGEM regulations. We also added a CAM resistance cassette.
The IGEM Plant Project
We hope to demonstrate that using biobricks in plants is not only useful but feasible in the restraints of this competition. We also hope to use the tools that we make to insert our toxin detection vector into plants.
Plant Protocol
Growing Plants
Selection of Primary Transformants
Transformation Protocol
Status of Project
Currently we have the multiple cloning site (MCS) and the vector that we are introducing it into (p0380). We have optimized the vector p0380 using site directed mutagenesis to remove all pre-existing notI sites from the vector. At the moment we are in the process of verifying that our MCS has been successfully inserted into the vector.
First Seed Pod of the project
