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Team:UCSF/Parts
From 2008.igem.org
AarI TOPO clones (digestion of these plasmids with AarI will release a fragment for AarI cloning).
GFP BD
Sir4
Sir2 AB
Sir2 BD
mCherry AB
mCherry BD
LexA (1-87) AB
LexA (1-87) BD
Pif3 AB
PhyB AB
Sas2 AB
Sas2 BD
AarI Shuttle Vector So you don't want to use AarI cloning? We've provided a shuttle vector that you can subclone an AarI AB or BD part into, then cut it back out with biobrick sites.
AarI--Biobrick Shuttle AB
AarI--Biobrick Shuttle BD
Bonus AarI Parts
These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.
Esa1 AB
AarI Acceptor Vectors
pRS315, Adh1P, Adh1t: CEN ARS vector for yeast expression (LEU2 marker), with Adh1 promoter and terminator.
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