Team:UCSF/Parts

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For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1


Project-relevant AarI TOPO clones

digestion of these plasmids with AarI will release a fragment for AarI cloning.

  • LexA (1-87 a.a) AB
  • Sir2 BD
  • GFP AD
  • Ssn8 AD
  • Sir3 A!D
  • Sir4 AD

GFP BD

Sir4

Sir2 AB


mCherry AB

mCherry BD


LexA (1-87) BD

Pif3 AB

PhyB AB

Sas2 AB

Sas2 BD

AarI acceptor vectors (empty)

These accept AarI digested parts, and include a promoter/terminator.

AarI Shuttle Vector

So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.

  • AarI--Biobrick Shuttle AB
  • AarI--Biobrick Shuttle BD


Bonus AarI Parts These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.

Esa1 AB




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