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Team:University of Sheffield/Rosie
From 2008.igem.org
| Introduction | Our project | Modelling | Wet Lab | Our team | Timetable | Miscellaneous |
|---|
| Introduction | Protocols |
|---|
Rosie's Lab Book
Early Work
Training, involving being familiarised with all the protocols listed with extensive practising
Biobrick Book Testing
After Eva's characterisation failed, we had our suspicions about the BioBrick Registry. However, given our own intermediate skill, and the prescence of many far more likely problems, it wasnt until we started to make the BioBricks that the full scale of the problem was obvious. After extractng and restriction the BioBrick vector pSB10A3, nothing showed up on the gel.
Lane1: Marker
Lanes 2 - 6: BBpSB1AK3 digested
Below is the gel of 5 extractions from BioBricks all over the registry (emphasis on vectors as thats waht we needed) - our final test to see if the book was faulty or not:
Lane 1: Marker
Lane 2: pSB1AK3
Lane 3: pSB1AC3 source plate:1020 well:4B
Lane 4: pSB1A2 sp:1003 w:4A
Lane 5: pBAD/araC sp:1013 w:5C
Lane 6: 30C6HSL reciever sp:1001 w:12B
Lane 7: Banana odour sp:1021 w:12B
As you can see, there is no DNA present.
