"
Cellular Communication Systems
From 2008.igem.org
Lux cell signaling system
Responsible: Andrew Gordon and Pallavi Penumetcha
Signal molecule: N-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules
Bacterial species: discovered in Vibrio fischeri known to work in E. coli
Receiver protein: LuxR protein receives signal from AHL; also has some control over transciption of luciferase
Signal molecule synthase: LuxI; also has some control over transciption of luciferase
Additional Information: "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration
Resources
[http://partsregistry.org/Lux Lux Operon Pathway]
[http://partsregistry.org/AHL AHL signaling molecules by species; some are specific to gram pos but may affect gram negs]
[http://www.horizonpress.com/jmmb/v1/v1n1/03.pdf Reducing Crosstalk in Lux System]
References
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522112 Quorum Quenching to control Lux Pathway]
[http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation
Parts Needed:
LuxR + pro/term
RBS
LuxI + pro/term
LuxI sender
