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Team:Chiba/Qurum Sensing Cross-talk
From 2008.igem.org
In this project, we use acylated homoserine lactones (AHLs), the signaling molecules used in [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] system among gram negative bacteria.
Senders express LuxI or its family enzymes, the producer of AHLs. LuxIs are placed under the control of constitutive (Tet) promoter; each cell generates AHL more or less in a constant rate. AHL can freely permeate the cell membranes and get detected by neighboring cells.
Receivers constitutively express LuxR proteins (or its homolog), the detector of AHLs. When AHLs bind the LuxR proteins, AHL-LuxR complex activate the Lux promoter on. The switching threshold (in [AHL]) is determined by the affinity between AHL and LuxR.
豊田も梅野さんの案で賛成
