Team:UCSF/Parts

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UCSF iGEM 2008 Parts

For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1


AarI Shuttle Vector The bulk of our parts/constructs were generated using the AarI cloning technique. If that is not your thing, we have created a shuttle vector to facilitate conversion of our parts into biobricks.

  • AarI--Biobrick Shuttle AB
  • AarI--Biobrick Shuttle BD

more details: Everything_you_ever_wanted_to_know_about_AarI


Project-relevant AarI Parts digestion of these plasmids with AarI will release a fragment for AarI cloning.

  • LexA (1-87 a.a) AB
  • Sir2 BD
  • GFP AD
  • Ssn8 AD
  • Sir3 A!D
  • Sir4 AD

Project-relevant Composite Parts AarI combinatorial cloning, combined with an occasional subclone, produced these composite parts.

EFFECTORS

  • LexA-Sir2 under a galactose-inducible promoter (Gal1P-LexA(1-87)-Sir2-Adh1t)
  • LexA-Sir2 under a strong constitutive promoter (Adh1P-LexA(1-87)-Sir2-Adh1t)
  • LexA-Sir2 under a medium constitutive promoter (Cyc1P-LexA(1-87)-Sir2-Adh1t)
  • Ssn8, under a strong constitutive promoter (Adh1P-Ssn8-Adh1t)
  • Sir3, under a strong constitutive promoter (Adh1P!-Sir3-Adh1t)

REPORTERS


AarI acceptor vectors (empty) These pRS3series vectors accept AarI digested parts between a promoter/terminator. The promoter is flanked by PspOMI/XhoI sites, and the terminator by Not1/SacI sites, in case you want to change them out.

  • AarI AD Acceptor (pRS305, Gal1P, Adh1t)
  • AarI AD Acceptor (pRS315, Adh1P, Adh1t)
  • AarI AD Acceptor (pRS315, Cyc1P, Adh1t)
  • AarI AD Acceptor (pRS315, Fig1P, Adh1t)
  • AarI AD Acceptor (pRS315, 8X LexA Ops-Cyc1P, Adh1t)
  • AarI AD acceptor (pRS315, Cyc1P, Adh1t-8XLexA Ops)
  • AarI AD Acceptor (pRS315, 8X LexA Ops-Fig1P, Adh1t)


Bonus AarI Parts These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.

Sir2 AB

Esa1 AB (Histone Acetyltransferase)

Sas2 AB (Histone Acetylatransferase)

Sas2 BD

mCherry AB

mCherry BD

GFP AB

GFP BD

LexA (1-87) BD

TetR AB (Tet Repressor DNA Binding Domain)

TetR BD

LacI AB (Lac Repressor DNA Binding Domain)

Pif3 AB (Light-inducible dimerization partner)

PhyB AB (Light-inducible dimerization partner)




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