Team:Chiba/Calendar-Home/16 September 2008

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15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

time-delay test with (15/9) cultures.

  • reaction temparature was 37°C and 30°C.)
  • result


  • Created Glycerol Stocks from (15/9) cultures.

Team:Output


Digestion test

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(×2)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(×5)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(×2)
  • [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
  • [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
  • [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
Sample No. each
DNA(μL) 3
EcoRI(μL) 0.083
PstI(μL) 0.083
BSA(×10)(μL) 1
Buffer3(×10)(μL) 1
dH2O(μL) 5
TOTAL(μL) 10

-->37°C 1 hour

Gel check

Sample No. each
DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12

Transformation

  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

PCR

  • Venus YFP(ptet+LuxR+YFP)
Sample No. YFP
DNA(μL) 1
Venus Fwd(μL) 5
Rev(VR)(μL) 5
Thermo pol Buffer(μL) 10
dNTP mix(μL) 10
Vent(μL) 1
dH2O(μL) 68
TOTAL(μL) 100
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