From 2008.igem.org
| We are the first Polish team starting in iGEM. We consist of University of Warsaw, Faculty of Biology students interested in molecular biology and genetic engineering, associated in students scientific societies.
The goal of our team is to make the biological machine based on E. coli cells able to produce specific monoclonal antibodies to given antigen
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Our research project is divided in three basic stages:
- In stage 1 we will introduce mechanism of targeted increase of genetical variability in regions of bacterial genome encoding antibodies. In this situation every bacterial cell in population will express different antibody on its surface.
- Goal of stage 2 is to provide selection mechanism, which will cause survival of the cells only when expressed antibody binds with target protein. Selection mechanism is based on antibiotic resistance induced by binding of target protein to antibody on cell surface.
- In stage 3 cellular metabolism will be switched to efficient antibody production and export.
Antibody will be then purified from culture medium. At all stages we have planed various control experiments, which will allow us to determine our progress.
This is very simplified description of our ‘biological machine’. Success of our project would allow simple, fast and cheap production of antibodies with desired specificity.
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