User:University of Washington/27 June 2008

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Contents

Yeast Shuttle Plasmid

- 1.5 mL 15% glycerol stocks of the overnight BY4741 yeast growth cultures were made by combining 563 uL of 40% glycerol and 937 uL yeast culture, then stored at -80 degrees Celsius.

Conjugation

Mini Prep
·R0042 (LuxR promoter)

Glycerol
·R0042 (LuxR promoter)

UW Glycerol Stocks

LuxR from AraC and TetR

- Made glycerol stock

- Did mini Prep

- Submitted DNA for sequencing reaction and analysis (only for AraC)


Team:University_of_Washington/Notebook#Notebook

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